Effectene transfection manual

Transfection using Effectene Reagent was optimized according to the manufacturer’s specifications for adherent cells in a mm dish (roughly the same surface area as a 25 cm2 flask). The DNA, buffer, and Enhancer were incubated for 5 min before adding the Effectene Reagent and incubated for 15 min to allow transfection complex www.doorway.ru Size: 97KB.  · In another study which compared the transfection outcome between different cell lines from human and animal origin, pig tracheal epithelial cells (PTE) was shown to be more efficiently transfected than the human tracheal epithelial cell line (HTE) by transfection reagents including Effectene, Lipofectamine Plus and PEI (Maurisse et al., ). Effectene Transfection Reagent Handbook 05/ 7 Introduction Effectene Transfection Reagent is a unique non-liposomal lipid formulation designed to achieve high transfection efficiencies. Effectene Transfection Reagent offers significant advantages over many liposome-based reagents and other transfection methods. Effectene Transfection Reagent means.

1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or transfections in well plates The Effectene Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. In another study which compared the transfection outcome between different cell lines from human and animal origin, pig tracheal epithelial cells (PTE) was shown to be more efficiently transfected than the human tracheal epithelial cell line (HTE) by transfection reagents including Effectene, Lipofectamine Plus and PEI (Maurisse et al., ). Effectene Transfection Reagent Handbook: www.doorway.ru Safety Data Sheets: www.doorway.ru Technical assistance: www.doorway.ru Notes before starting This protocol is for transient or stable transfection of adherent cells in 6-well plates. For transfection using other culture formats, refer to Table 1.

1 ឧសភា Effectene Transfection Reagent Handbook 05/ 4. Primary Cells and Cell Lines. Adherent cells. Suspension cells. used in the Drosophila community since techniques for their transfection were Cellfectin, FuGene 6, and Effectene (Qiagen) on about 20 cell lines of. Each reagent was tested with a range of transfection reagent:DNA ratios and two to DNA ratios were tested according to the manufacturer's instructions.